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Protein function (1989)

Public ISBD Titre : Protein function : A Practical Approach Type de document : texte imprimé Auteurs : Thomas E. Creighton (1940-....), Éditeur scientifique Editeur : Oxford : IRL Press Année de publication : 1989 Collection : Practical approach series, ISSN 0957-025X Importance : 306 p. Format : 23 cm ISBN/ISSN/EAN : 978-1-85221-139-4 Langues : Anglais (eng) Tags : Proteins Index. décimale : 547.7 Résumé : The analysis of protein function is a vital step in the characterization of any newly discovered protein. This new edition brings up to date the techniques used, and presents experimental procedures that can be performed in the average laboratory without recourse to highly specialised equipment. The protocols will be of use to both experienced and novice researchers and are accompanied by background information, hints and tips, and troubleshooting guides to ensure successful elucidation of protein function. Note de contenu : "This edition . . . has been updated from the original 1989 edition . . . [N]ew chapters have . . . been added to reflect recent improvements in the techniques of molecular biology. . . . The ten chapters roughly focus on five areas. One of the main topics is the chemical modification of proteins . . . Another section considers techniques involved in the detection and quantification of ligand-receptor binding interactions. A third focuses on the purification and analysis of sequence-specific DNA binding proteins. . . . One of the new additions is a discussion of the techniques involved in the overexpression of cloned genes in E. coli and of those used in protein refolding. These chapters are particularly useful, as they contain warnings of numerous pitfalls as well as valuable suggestions that would aid researchers new to these areas. A final addition is a chapter on the identification of proteins by using two-dimensional electrophoresis." Protein function : A Practical Approach [texte imprimé] / Thomas E. Creighton (1940-....), Éditeur scientifique . - Oxford : IRL Press, 1989 . - 306 p. ; 23 cm. - (Practical approach series, ISSN 0957-025X) . ISBN : 978-1-85221-139-4 Langues : Anglais (eng) Tags : Proteins Index. décimale : 547.7 Résumé : The analysis of protein function is a vital step in the characterization of any newly discovered protein. This new edition brings up to date the techniques used, and presents experimental procedures that can be performed in the average laboratory without recourse to highly specialised equipment. The protocols will be of use to both experienced and novice researchers and are accompanied by background information, hints and tips, and troubleshooting guides to ensure successful elucidation of protein function. Note de contenu : "This edition . . . has been updated from the original 1989 edition . . . [N]ew chapters have . . . been added to reflect recent improvements in the techniques of molecular biology. . . . The ten chapters roughly focus on five areas. One of the main topics is the chemical modification of proteins . . . Another section considers techniques involved in the detection and quantification of ligand-receptor binding interactions. A third focuses on the purification and analysis of sequence-specific DNA binding proteins. . . . One of the new additions is a discussion of the techniques involved in the overexpression of cloned genes in E. coli and of those used in protein refolding. These chapters are particularly useful, as they contain warnings of numerous pitfalls as well as valuable suggestions that would aid researchers new to these areas. A final addition is a chapter on the identification of proteins by using two-dimensional electrophoresis."

Exemplaires (1)

Code-barres	Cote	Support	Localisation	Section	Disponibilité
01896	20bm-02-37	livres	Bibliothèque de la faculté S.N.V * HARCHE MERIEM*	livres	Consultation sur place Exclu du prêt

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