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Titre : Microarray Technology and Its Applications Type de document : texte imprimé Auteurs : Uwe R. , Auteur ; Dan V Nicolau, Directeur de publication ; Dan V. Nicolau Mention d'édition : 1st ed. 2005. Editeur : Berlin, Heidelberg : Springer Berlin Heidelberg Année de publication : 2005 Collection : Biological and medical physics, biomedical engineering (Internet), ISSN 2197-5647 ISBN/ISSN/EAN : 978-3-540-26578-8 Langues : Anglais (eng) Tags : Biomedicine Biophysics/Biomedical Physics Biomedical Engineering Biomedicine general Nanotechnology Physical Chemistry Medicine/Public Health, general Biomedical Engineering and Bioengineering. Biological and Medical Physics, Biophysics. Index. décimale : 610 Médecine - hygiène, santé Résumé : The genomics revolution would not have been possible without the 'parallelisation' offered by microarray technology. This technological - and commercial - success has been since emulated by other applications areas, with a tremendous amplification of innovation. This book describes the fundamentals and latest developments in microarray technology, as well as its future directions. It presents detailed overviews of the different techniques of fabricating microarrays, of the chemistries and preparative steps involved, of the different types of microarrays, and of the instrumentation and optical issues involved Note de contenu : General Microarray Technologies
Array Formats
Biomolecules and Cells on Surfaces [xe2][x80][x94] Fundamental Concepts
Surfaces and Substrates
Reagent Jetting Based Deposition Technologies for Array Construction
Manufacturing of 2-D Arrays by Pin-printing Technologies
Nanoarrays
The Use of Microfluidic Techniques in Microarray Applications
Labels and Detection Methods
Marker-free Detection on Microarrays
DNA Microarrays
Analysis of DNA Sequence Variation in the Microarray Format
High Sensitivity Expression Profiling
Applications of Matrix-CGH (Array-CGH) for Genomic Research and Clinical Diagnostics
Analysis of Gene Regulatory Circuits
Protein Microarrays
Protein, Antibody and Small Molecule Microarrays
Photoaptamer Arrays for Proteomics Applications
Biological Membrane Microarrays
Cell & Tissue Microarrays
Use of Reporter Systems for Reverse Transfection Cell Arrays
Whole Cell Microarrays
Tissue Microarrays for Miniaturized High-Throughput Molecular Profiling of Tumors
Application of Microarray Technologies for Translational GenomicsEn ligne : http://docelec.u-bordeaux.fr/login?url=http://www.springerlink.com/openurl.asp?g [...] Microarray Technology and Its Applications [texte imprimé] / Uwe R. , Auteur ; Dan V Nicolau, Directeur de publication ; Dan V. Nicolau . - 1st ed. 2005. . - Berlin, Heidelberg : Springer Berlin Heidelberg, 2005. - (Biological and medical physics, biomedical engineering (Internet), ISSN 2197-5647) .
ISBN : 978-3-540-26578-8
Langues : Anglais (eng)
Tags : Biomedicine Biophysics/Biomedical Physics Biomedical Engineering Biomedicine general Nanotechnology Physical Chemistry Medicine/Public Health, general Biomedical Engineering and Bioengineering. Biological and Medical Physics, Biophysics. Index. décimale : 610 Médecine - hygiène, santé Résumé : The genomics revolution would not have been possible without the 'parallelisation' offered by microarray technology. This technological - and commercial - success has been since emulated by other applications areas, with a tremendous amplification of innovation. This book describes the fundamentals and latest developments in microarray technology, as well as its future directions. It presents detailed overviews of the different techniques of fabricating microarrays, of the chemistries and preparative steps involved, of the different types of microarrays, and of the instrumentation and optical issues involved Note de contenu : General Microarray Technologies
Array Formats
Biomolecules and Cells on Surfaces [xe2][x80][x94] Fundamental Concepts
Surfaces and Substrates
Reagent Jetting Based Deposition Technologies for Array Construction
Manufacturing of 2-D Arrays by Pin-printing Technologies
Nanoarrays
The Use of Microfluidic Techniques in Microarray Applications
Labels and Detection Methods
Marker-free Detection on Microarrays
DNA Microarrays
Analysis of DNA Sequence Variation in the Microarray Format
High Sensitivity Expression Profiling
Applications of Matrix-CGH (Array-CGH) for Genomic Research and Clinical Diagnostics
Analysis of Gene Regulatory Circuits
Protein Microarrays
Protein, Antibody and Small Molecule Microarrays
Photoaptamer Arrays for Proteomics Applications
Biological Membrane Microarrays
Cell & Tissue Microarrays
Use of Reporter Systems for Reverse Transfection Cell Arrays
Whole Cell Microarrays
Tissue Microarrays for Miniaturized High-Throughput Molecular Profiling of Tumors
Application of Microarray Technologies for Translational GenomicsEn ligne : http://docelec.u-bordeaux.fr/login?url=http://www.springerlink.com/openurl.asp?g [...] Exemplaires (2)
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Titre : Cell Motility Type de document : texte imprimé Auteurs : Peter Lenz, Auteur Mention d'édition : 1st ed. 2008. Editeur : New York, NY : Springer New York Année de publication : 2008 Collection : Biological and medical physics, biomedical engineering (Internet), ISSN 2197-5647 Importance : 248 p. Présentation : ill Format : 25 cm ISBN/ISSN/EAN : 978-0-387-73049-3 Note générale : index 237p-248p. Langues : Anglais (eng) Tags : Physics Biophysics/Biomedical Physics Freshwater & Marine Ecology Biomedical Engineering Cell Biology Human Physiology Biomedical Engineering and Bioengineering. Biological and Medical Physics, Biophysics. Index. décimale : 571.4 Résumé : Cell motility is a fascinating example of cell behavior which is fundamentally important to a number of biological and pathological processes. It is based on a complex self-organized mechano-chemical machine consisting of cytoskeletal filaments and molecular motors. This network is highly dynamic, but able to show precise spatial and temporal organization. The machine is regulated by a complex network of biochemical reactions coupled to force and movement generating processes.
In general, the cytoskeleton is responsible for the movement of the entire cell and for movements within the cell. There are two ways by which cells can move: swimming (i.e. movement through liquid water) and crawling (i.e. movement across a rigid surface). Swimming cells experience viscous forces that are orders of magnitude greater than inertial forces. Therefore, swimming cells undergo an non-symmetric (i.e. non-reciprocal) sequence of shape changes. While for many bacterial cells motion is caused by the rotation of flagella, most swimming eukaryotic cells use the beating of hairlike extensions (such as cilia) to propel themselves through the liquid.
The movement of cells across rigid surfaces is even more complex. Here, one has to distinguish between crawling and gliding. In crawling motility, a cell (attached to a rigid substrate) extends forward a projection at its leading edge that then attaches to the substrate. There are 3 types of projections (filopodia, lamellipodia and pseudopodia) which are all filled with assemblies of cytoskeletal actin filaments. After protrusion and attachment, the crawling cell then contracts to move the cell body forward, and movement continues as a tread-milling cycle of front protrusion and rear retraction. Gliding cells slide across a rigid substrate by various mechanisms. The most important examples include jet propulsion, twitching, and dynamic organization of the pellicle (i.e. the skin of the cell).
Of biological importance are not only the movements of the cell as whole but also movements within the cell boundaries. For example, during mitosis the replicated chromosomes are cleaved and pulled to opposite poles of the cell by the mitotic spindle. Not only chromosomes, but also many other large molecules must be moved to specific locations within the cell. This can be achieved with active transport by molecular motors which move along cytoskeletal filaments. This motion is much more precise and quicker than diffusional motion. Motor proteins are essential for many processes of cellular motion. There is a whole variety of different motors. The most important classes include: linear motors (such as myosin, kinesin and dynein), rotatory motors (such as ATP synthase and bacterial flagella), and nucleic acid motors (such as helicases and topoisomerases). The linear motors use ATP to move along filaments. But they are much more than simple transporters. Two headed motors attach to adjacent filaments leading to sliding of oppositely oriented filaments (which is responsible for, e.g., muscle contraction). These induced interactions give rise to a complex cooperative behavior of collections of motors allowing cells to actively deform their shape.
On the other hand, single motors can exhibit more complex shape changes. For example, ATPsynthase (the motor which produces ATP) performs a rotational motion. While the biological function of the fluid flow generated by this motor is so far not understood, other rotatory motors enable bacteria to swim. For example, the flagellum of E.coli uses an ion flux to drive its rotation.Note de contenu : The Physics Of Listeria Propulsion
Biophysical Aspects of Actin-Based Cell Motility in Fish Epithelial Keratocytes
Directed Motility and Dictyostelium Aggregation
Microtubule Forces and Organization
Mechanisms of Molecular Motor Action and Inaction
Molecular Mechanism of Mycoplasma Gliding - A Novel Cell Motility System
Hydrodynamics and Rheology of Active Polar Filaments
Collective Effects in Arrays of Cilia and Rotational MotorsEn ligne : https://doi.org/10.1007/978-0-387-73050-9 Format de la ressource électronique : Cell Motility [texte imprimé] / Peter Lenz, Auteur . - 1st ed. 2008. . - New York, NY : Springer New York, 2008 . - 248 p. : ill ; 25 cm. - (Biological and medical physics, biomedical engineering (Internet), ISSN 2197-5647) .
ISBN : 978-0-387-73049-3
index 237p-248p.
Langues : Anglais (eng)
Tags : Physics Biophysics/Biomedical Physics Freshwater & Marine Ecology Biomedical Engineering Cell Biology Human Physiology Biomedical Engineering and Bioengineering. Biological and Medical Physics, Biophysics. Index. décimale : 571.4 Résumé : Cell motility is a fascinating example of cell behavior which is fundamentally important to a number of biological and pathological processes. It is based on a complex self-organized mechano-chemical machine consisting of cytoskeletal filaments and molecular motors. This network is highly dynamic, but able to show precise spatial and temporal organization. The machine is regulated by a complex network of biochemical reactions coupled to force and movement generating processes.
In general, the cytoskeleton is responsible for the movement of the entire cell and for movements within the cell. There are two ways by which cells can move: swimming (i.e. movement through liquid water) and crawling (i.e. movement across a rigid surface). Swimming cells experience viscous forces that are orders of magnitude greater than inertial forces. Therefore, swimming cells undergo an non-symmetric (i.e. non-reciprocal) sequence of shape changes. While for many bacterial cells motion is caused by the rotation of flagella, most swimming eukaryotic cells use the beating of hairlike extensions (such as cilia) to propel themselves through the liquid.
The movement of cells across rigid surfaces is even more complex. Here, one has to distinguish between crawling and gliding. In crawling motility, a cell (attached to a rigid substrate) extends forward a projection at its leading edge that then attaches to the substrate. There are 3 types of projections (filopodia, lamellipodia and pseudopodia) which are all filled with assemblies of cytoskeletal actin filaments. After protrusion and attachment, the crawling cell then contracts to move the cell body forward, and movement continues as a tread-milling cycle of front protrusion and rear retraction. Gliding cells slide across a rigid substrate by various mechanisms. The most important examples include jet propulsion, twitching, and dynamic organization of the pellicle (i.e. the skin of the cell).
Of biological importance are not only the movements of the cell as whole but also movements within the cell boundaries. For example, during mitosis the replicated chromosomes are cleaved and pulled to opposite poles of the cell by the mitotic spindle. Not only chromosomes, but also many other large molecules must be moved to specific locations within the cell. This can be achieved with active transport by molecular motors which move along cytoskeletal filaments. This motion is much more precise and quicker than diffusional motion. Motor proteins are essential for many processes of cellular motion. There is a whole variety of different motors. The most important classes include: linear motors (such as myosin, kinesin and dynein), rotatory motors (such as ATP synthase and bacterial flagella), and nucleic acid motors (such as helicases and topoisomerases). The linear motors use ATP to move along filaments. But they are much more than simple transporters. Two headed motors attach to adjacent filaments leading to sliding of oppositely oriented filaments (which is responsible for, e.g., muscle contraction). These induced interactions give rise to a complex cooperative behavior of collections of motors allowing cells to actively deform their shape.
On the other hand, single motors can exhibit more complex shape changes. For example, ATPsynthase (the motor which produces ATP) performs a rotational motion. While the biological function of the fluid flow generated by this motor is so far not understood, other rotatory motors enable bacteria to swim. For example, the flagellum of E.coli uses an ion flux to drive its rotation.Note de contenu : The Physics Of Listeria Propulsion
Biophysical Aspects of Actin-Based Cell Motility in Fish Epithelial Keratocytes
Directed Motility and Dictyostelium Aggregation
Microtubule Forces and Organization
Mechanisms of Molecular Motor Action and Inaction
Molecular Mechanism of Mycoplasma Gliding - A Novel Cell Motility System
Hydrodynamics and Rheology of Active Polar Filaments
Collective Effects in Arrays of Cilia and Rotational MotorsEn ligne : https://doi.org/10.1007/978-0-387-73050-9 Format de la ressource électronique : Exemplaires (2)
Code-barres Cote Support Localisation Section Disponibilité 02590 20bc-01-25 livres Bibliothèque de la faculté S.N.V * HARCHE MERIEM* livres Consultation sur place
Exclu du prêt02591 20bc-01-25 livres Bibliothèque de la faculté S.N.V * HARCHE MERIEM* livres prêt possible
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Bibliothèque de la Faculté SNV "HARCHE MERIEM"
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Bibliothèque de la Faculté SNV "HARCHE MERIEM"Faculté des sciences de la nature et de la vie
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