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Characterization of proteins (1988)
Titre : Characterization of proteins Type de document : texte imprimé Auteurs : Felix Franks, Éditeur scientifique Editeur : Chifton [new jersey] : Humana press Année de publication : 1988 Collection : Biological methods Importance : 1 vol. (xix-561 p) Présentation : ill Format : 23 cm ISBN/ISSN/EAN : 978-0-89603-109-8 Langues : Anglais (eng) Tags : Proteins Index. décimale : 574.1 Résumé : Proteins are the servants of life. They occur in all component parts of living organisms and are staggering in their functional var- ty, despite their chemical similarity. Even the simplest single-cell organism contains a thousand different proteins, fulfilling a wide range of life-supporting roles. Their production is controlled by the cell's genetic machinery, and a malfunction of even one protein in the cell will give rise to pathological symptoms. Additions to the total number of known proteins are constantly being made on an increasing scale through the discovery of mutant strains or their production by genetic manipulation; this latter technology has become known as protein engineering. The in vivo functioning of proteins depends critically on the chemical structure of individual peptide chains, but also on the detailed folding of the chains themselves and on their assembly into larger supramolecular structures. The molecules and their fu- tional assemblies possess a limited in vitro stability. Special methods are required for their intact isolation from the source material and for their analysis, both qualitatively and quantitatively. Proteins are also increasingly used as "industrial components," e.g., in biosensors and immobilized enzymes, because of their specificity, selectivity, and sensitivity. This requires novel and refined proce- ing methods by which the protein isolate can be converted into a form in which it can be utilized. Characterization of proteins [texte imprimé] / Felix Franks, Éditeur scientifique . - Chifton [new jersey] : Humana press, 1988 . - 1 vol. (xix-561 p) : ill ; 23 cm. - (Biological methods) .
ISBN : 978-0-89603-109-8
Langues : Anglais (eng)
Tags : Proteins Index. décimale : 574.1 Résumé : Proteins are the servants of life. They occur in all component parts of living organisms and are staggering in their functional var- ty, despite their chemical similarity. Even the simplest single-cell organism contains a thousand different proteins, fulfilling a wide range of life-supporting roles. Their production is controlled by the cell's genetic machinery, and a malfunction of even one protein in the cell will give rise to pathological symptoms. Additions to the total number of known proteins are constantly being made on an increasing scale through the discovery of mutant strains or their production by genetic manipulation; this latter technology has become known as protein engineering. The in vivo functioning of proteins depends critically on the chemical structure of individual peptide chains, but also on the detailed folding of the chains themselves and on their assembly into larger supramolecular structures. The molecules and their fu- tional assemblies possess a limited in vitro stability. Special methods are required for their intact isolation from the source material and for their analysis, both qualitatively and quantitatively. Proteins are also increasingly used as "industrial components," e.g., in biosensors and immobilized enzymes, because of their specificity, selectivity, and sensitivity. This requires novel and refined proce- ing methods by which the protein isolate can be converted into a form in which it can be utilized. Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité 01746 biotechno-53 livres Bibliothèque de la faculté S.N.V * HARCHE MERIEM* livres Consultation sur place
Exclu du prêtProtein function (1989)
Titre : Protein function : A Practical Approach Type de document : texte imprimé Auteurs : Thomas E. Creighton (1940-....), Éditeur scientifique Editeur : Oxford : IRL Press Année de publication : 1989 Collection : Practical approach series, ISSN 0957-025X Importance : 306 p. Format : 23 cm ISBN/ISSN/EAN : 978-1-85221-139-4 Langues : Anglais (eng) Tags : Proteins Index. décimale : 547.7 Résumé : The analysis of protein function is a vital step in the characterization of any newly discovered protein. This new edition brings up to date the techniques used, and presents experimental procedures that can be performed in the average laboratory without recourse to highly specialised
equipment. The protocols will be of use to both experienced and novice researchers and are accompanied by background information, hints and tips, and troubleshooting guides to ensure successful elucidation of protein function.Note de contenu : "This edition . . . has been updated from the original 1989 edition . . . [N]ew chapters have . . . been added to reflect recent improvements in the techniques of molecular biology. . . . The ten chapters roughly focus on five areas. One of the main topics is the chemical modification of proteins .
. . Another section considers techniques involved in the detection and quantification of ligand-receptor binding interactions. A third focuses on the purification and analysis of sequence-specific DNA binding proteins. . . . One of the new additions is a discussion of the techniques involved in the
overexpression of cloned genes in E. coli and of those used in protein refolding. These chapters are particularly useful, as they contain warnings of numerous pitfalls as well as valuable suggestions that would aid researchers new to these areas. A final addition is a chapter on the identification
of proteins by using two-dimensional electrophoresis."Protein function : A Practical Approach [texte imprimé] / Thomas E. Creighton (1940-....), Éditeur scientifique . - Oxford : IRL Press, 1989 . - 306 p. ; 23 cm. - (Practical approach series, ISSN 0957-025X) .
ISBN : 978-1-85221-139-4
Langues : Anglais (eng)
Tags : Proteins Index. décimale : 547.7 Résumé : The analysis of protein function is a vital step in the characterization of any newly discovered protein. This new edition brings up to date the techniques used, and presents experimental procedures that can be performed in the average laboratory without recourse to highly specialised
equipment. The protocols will be of use to both experienced and novice researchers and are accompanied by background information, hints and tips, and troubleshooting guides to ensure successful elucidation of protein function.Note de contenu : "This edition . . . has been updated from the original 1989 edition . . . [N]ew chapters have . . . been added to reflect recent improvements in the techniques of molecular biology. . . . The ten chapters roughly focus on five areas. One of the main topics is the chemical modification of proteins .
. . Another section considers techniques involved in the detection and quantification of ligand-receptor binding interactions. A third focuses on the purification and analysis of sequence-specific DNA binding proteins. . . . One of the new additions is a discussion of the techniques involved in the
overexpression of cloned genes in E. coli and of those used in protein refolding. These chapters are particularly useful, as they contain warnings of numerous pitfalls as well as valuable suggestions that would aid researchers new to these areas. A final addition is a chapter on the identification
of proteins by using two-dimensional electrophoresis."Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité 01896 20bm-02-37 livres Bibliothèque de la faculté S.N.V * HARCHE MERIEM* livres Consultation sur place
Exclu du prêt
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